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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in advertising and marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two further splice variants were being described but in depth nucleotide sequence information will not be but available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded from the antibody.
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This guideline outlines further more probable optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
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This guidebook outlines further feasible optimization of cellular and immunoassay parameters to be sure the absolute best results are...
Antibodies sure to the blocking peptide not bind towards the epitope around the focus on protein. By comparing the staining from the blocked antibody compared to the antibody on your own, one can see which staining is unique.
Concentrate on Details Cyclin E belongs into the very conserved cyclin spouse and children, whose members are characterised by a spectacular periodicity in protein abundance through the here mobile cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add into the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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